Lag, Log, Stationary or Death phase and the Culture Conditions. The number of cells per ml will depend on the phase of culture, i.e. Introduction Bacterial cultures can have millions of cells per milliliter. Serial Dilution Lab Activity Serial Dilution Lab Report ConclusionĢ) If the number of bacteria in the samples is high, colonies of bacteria are likely to cover the whole plate so that one cannot count any colonies at all. Lab Exercise 7: Enumerations of the total number of bacteria found in a given culture. Serial dilution is a useful technique because the colonies are counted twice with different people and the average number of colonies is reported as the number of bacteria present in the sample after multiplying the dilution factor. What are two reasons serial dilution is a useful technique for counting populations of bacteria? ) When there are a lot of bacteria, it is hard to count undiluted colonies accurately because there could be hundreds of them. This experiment is carried out by a serial dilution. Some of these may include lactic acid bacteria, coliforms, clostridium, streptococcus, bacillus cereus, salmonella, Escherichia coli O157, and campylobacter. What was the approximate concentration of the original sample? This lab report comprises of an investigation into the different microorganisms present in a diary product milk. 4 x 1010 cells/ml 4.Ī 1 ml sample of bacteria was diluted to 10-6 and 100? L was plated on the Petri dish at right. Experiment 16 Preview text Experiment 34 An Equilibrium Constant CHEM-1112-04 The University of Texas Rio Grande Valley Spring 2019 Swati Mohan Emilio Molina Objective: The experiment performed was meant to determine the equilibrium constant of a chemical system by using a spectrophotometer. The number of viable bacteria in the original sample was a. Then 4 serial 10-fold dilutions are made. You dilute 1 ml of an unknown sample of bacteria to 1 x 10-6 and plate out 100? L onto a Petri dish. Example 2: 1 ml of a bacterial culture is transferred to 9.0 ml of diluent. For only $13.90/page You make six 2-fold serial dilutions of a solution.
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